UCA1 executes an oncogenic role in pancreatic cancer by regulating miR-582-5p/BRCC3

Background As a fatal disease, the mechanism of pancreatic cancer is unclear. Urothelial carcinoma antigen 1(UCA1), long noncoding RNA (lncRNA) that was first reported in bladder cancer, acts as an oncogene. However, regulatory role and UCA1 remain unknown. This study aims to investigate expression level prognostic value tissues, effects regulating cell proliferation, apoptosis metastasis. Methods levels tissues were detected by situ hybridization (ISH) evaluated univariate multivariate survival analysis. For vitro experiments, proliferation count kit assay, Edu clone formation assay. Apoptosis fluorescence-activated sorting flow-cytometry. Cell migration invasion capacities wound healing transwell assays. Western blots performed detect apoptotic associated molecules epithelial-mesenchymal transition (EMT) markers. vivo experiment, subcutaneous transplantation models nude mice established observe tumor growth. The explored proteomics, bioinformatic analysis, luciferase reporter assays, rescue experiments. Results ISH staining revealed between (n=94) tumor-adjacent (n=73) did not show significant differences. Survival analysis indicated high unfavorable prognosis factor for cancer. Downregulation siRNA significantly inhibited decreased invasion, induced apoptosis, EMT. Furthermore, we demonstrated positively regulated BRCC3 inhibiting miR-582-5p. Rescue experiments either miR-582-5p or enhancing could partly attenuate antitumor downregulation UCA1. Conclusion acted oncogene miR-582-5p/BRCC3, which be new therapeutic target